教員紹介

伊原　誠
准教授

所属	農学部応用生命化学科農学研究科
学位	博士（農学）
専門	農薬化学
コメント	イオンチャネルを標的とする農薬の作用機構を研究しています。特に、ネオニコチノイド剤の昆虫選択的毒性発現の分子機構を、構造生理学的観点から重点的に解析しています。
リサーチマップリンク	https://researchmap.jp/makoto.ihara

研究室ホームページへ

学歴/経歴

経歴

2018年4月 - 現在
近畿大学農学部准教授
2013年4月 - 2018年3月
近畿大学農学部応用生命化学科講師
2012年12月 - 2018年3月
独立行政法人理化学研究所放射光科学総合研究センター客員研究員
2012年12月 - 2013年3月
岡山大学大学院医歯薬学総合研究科（薬学系）助教
2006年10月 - 2012年11月
独立行政法人理化学研究所播磨研究所放射光科学総合研究センター研究員
2005年4月 - 2006年9月
近畿大学研究員

研究活動情報

研究分野

ライフサイエンス, 生物有機化学
ライフサイエンス, 機能生物化学
ライフサイエンス, 構造生物化学
ナノテク・材料, 生物分子化学

研究キーワード

生物有機化学, 農薬科学, 構造生理学, Structural Biology

論文

Divergent Nine-Step Syntheses of Perhydrohistrionicotoxin Analogs and Their Inhibition Activity Toward Chicken α4β2-Neuronal Nicotinic Acetylcholine Receptors. , Keisuke Nishikawa; Yosuke Ono; Sumito Mori; Koichi Takayama; Makoto Ihara; Kazuhiko Matsuda; Yoshiki Morimoto , The Journal of organic chemistry , 89 , 6 , 4128-4133 , 2024年3月15日 , 査読有り
Creating Pyrethrin Mimetic Phosphonates as Chemical Genetics Tools Targeting the GDSL Esterase/Lipase TcGLIP to Investigate Pyrethrin Biosynthesis. , Noritada Matsuo; Yukimi Sugisaka; Shiori Aoyama; Makoto Ihara; Harue Shinoyama; Munetaka Hosokawa; Yoshinobu Kamakura; Daisuke Tanaka; Yoo Tanabe; Kazuhiko Matsuda , Journal of medicinal chemistry , 66 , 12 , 7959-7968 , 2023年6月22日 , 査読有り
Determinants of Subtype-Selectivity of the Anthelmintic Paraherquamide A on Caenorhabditis elegans Nicotinic Acetylcholine Receptors. , Wataru Koizumi; Shuya Otsubo; Shogo Furutani; Kunihiro Niki; Koichi Takayama; Shota Fujimura; Takanobu Maekawa; Ryosuke Koyari; Makoto Ihara; Kenji Kai; Hideo Hayashi; Mohammad Shaokat Ali; Eriko Kage-Nakadai; David B Sattelle; Kazuhiko Matsuda , Molecular pharmacology , 103 , 6 , 299-310 , 2023年6月 , 査読有り

セイヨウミツバチのニコチン性アセチルコリン受容体の機能的発現と薬理特性に対する補助因子の効果 , 伊原誠; 高山浩一; 伊藤稜; 森澄海人; 武林真由花; 江川瑠夏; 山本新菜; 木村裕紀; 松田一彦 , 日本農芸化学会2024年度大会 , 2024年3月25日
Paraherquamide A の C. elegans ニコチン性アセチルコリン受容体サブタイプ選択性の分子機構 , 伊原誠; 大坪柊也; 高山浩一; 松田一彦 , 2023 年度日本農芸化学会中部・関西支部合同大会（中部支部：第 196 回例会）（関西支部：第 527 回講演会） , 2023年10月1日
Determinants of sub-type selectivity of Paraherquamide A on Caenorhabditis elegans nicotinic acetylcholine receptors , Makoto Ihara , Control of Human Disease Vectors, Pests and Parasites , 2023年9月

Ligand-gated ion channels as targets of neuroactive insecticides. , Makoto Ihara , Bioscience, biotechnology, and biochemistry , 86 , 2 , 157 , 164 , 2022年1月24日
概要：The Cys-loop superfamily of ligand-gated ion channels (Cys-loop receptors) is one of the most ubiquitous ion channel families in vertebrates and invertebrates. Despite their ubiquity, they are targeted by several classes of pesticides, including neonicotinoids, phenylpyrazols, and macrolides such as ivermectins. The current commercialized compounds have high target site selectivity, which contributes to the safety of insecticide use. Structural analyses have accelerated progress in this field; notably, the X-ray crystal structures of acetylcholine binding protein and glutamate-gated Cl channels revealed the details of the molecular interactions between insecticides and their targets. Recently, the functional expression of the insect nicotinic acetylcholine receptor (nAChR) has been described, and detailed evaluations using the insect nAChR have emerged. This review discusses the basic concepts and the current insights into the molecular mechanisms of neuroactive insecticides targeting the ligand-gated ion channels, particularly Cys-loop receptors, and presents insights into target-based selectivity, resistance, and future drug design.
Neonicotinoid Insecticides: Molecular Targets, Resistance, and Toxicity. , Kazuhiko Matsuda; Makoto Ihara; David B Sattelle , Annual review of pharmacology and toxicology , 60 , 241 , 255 , 2020年1月6日
概要：Copyright © 2020 by Annual Reviews. All rights reserved. Neonicotinoids have been used to protect crops and animals from insect pests since the 1990s, but there are concerns regarding their adverse effects on nontarget organisms, notably on bees. Enhanced resistance to neonicotinoids in pests is becoming well documented. We address the current understanding of neonicotinoid target site interactions, selectivity, and metabolism not only in pests but also in beneficial insects such as bees. The findings are relevant to the management of both neonicotinoids and the new generation of pesticides targeting insect nicotinic acetylcholine receptors.
Neonicotinoids: molecular mechanisms of action, insights into resistance and impact on pollinators. , Makoto Ihara; Kazuhiko Matsuda , Current opinion in insect science , 30 , 86 , 92 , 2018年12月
概要：© 2018 Elsevier Inc. Neonicotinoids are insecticides that target insect nicotinic acetylcholine receptors (nAChRs), exhibiting high selective toxicity to insects over vertebrates and good systemic activity in crop plants. For these reasons, neonicotinoids currently make up ∼30% of insecticide sales worldwide. However, due to their adverse impact on pollinators such as honey bees and bumble bees, neonicotinoids are being banned from the EU, and other countries may follow. It is therefore crucial to understand the mechanism underlying neonicotinoid actions on pollinators as well as on the nAChRs of pests, with a view to understanding their selectivity. Here we review the molecular mechanisms of neonicotinoid actions at an atomic level, through structural and resistance mechanism studies and propose relevant research topics for further studies on the future of pest management.