教員紹介

加藤　容子
教授

所属	農学部生物機能科学科農学研究科
学位	博士（農学）
専門	動物発生工学
コメント	哺乳動物細胞核の全能性や多能性の誘導ならびに卵子の初期化機構の解明に興味を持ち、長年取り組んでいます。また、卵子や初期胚の凍結保存や新たな保存法の開発も行っています。
リサーチマップリンク	https://researchmap.jp/AnimalReprod

学歴/経歴

経歴

2009年 - 現在
近畿大学農学部教授

研究活動情報

研究分野

ライフサイエンス, 実験動物学
ライフサイエンス, 動物生産科学

論文

Rescue of oocytes recovered from postmortem mouse ovaries. , Nagi Fujii; Yuta Nakata; Yoko Kato , Experimental animals , 2022年8月15日 , 査読有り
Gamete and Embryo Technology: Cloning , Yoko Kato , Encyclopedia of Dairy Sciences (Third edition) , 868-873 , 2022年 , 査読有り , 招待有り
Effect of phytohemagglutinin (PHA) on nuclear transfer pig embryos in vitro , Ndubuisi Machebe; Soichiro Shimizu; Masaki Hata; Kazuki Ohata; Tetsuya Tani; Yoko Kato , Journal of Mammalian Ova Research , 36 , 33-43 , 2019年4月 , 査読有り

p53 遺伝子がもたらすエピブラスト様細胞への誘導機構 , 青木雪来; 加藤容子; 岡村大治 , 第３９回日本受精着床学会 , 2021年7月15日
家畜繁殖学研究の最先端～基礎から臨床へ～ , 加藤容子 , 第39回日本受精着床学会 , 2021年7月15日
GV期核置換卵の生存性向上に関わる要因 , 真柄怜央; 加藤容子 , 第６２回日本卵子学会 , 2021年5月29日

〈Original〉 Effects of FGF9 on porcine in vitro maturation and development after parthenogenetic activation , アニエクェ, ・チオマ・ジェーン; 前山, 怜; 真柄, 怜央; 青木, 雪来; 東野, 愛理; 加藤, 容子 , 近畿大学農学部紀要 = MEMOIRS OF THE FACULTY OF AGRICULTURE OF KINDAI UNIVERSITY , 55 , 15 , 24 , 2022年3月31日
概要：type:Departmental Bulletin Paper [Synopsis] Fibroblast growth factor 9 (FGF9) plays a key role in the regulation of cell differentiation, cell proliferation and embryonic development. It is also a survival factor for various cell types and interacts with two FGF receptors, FGFR2 and FGFR3. Furthermore, the absence of specific thecal-and oocyte-derived products during in vitro maturation (IVM) is suggested to be one reason for the reductions in the competency of oocytes used in assisted reproductive techniques. FGF9, as with some FGFs shown to promote oocyte competence and embryo development in several species is localize largely in theca cells, somewhat in the oocyte and its receptors in oocytes and cumulus cells. Therefore, to investigate the possible reproductiverelated implications of FGF9 supplementation, its effects on IVM oocytes after parthenogenetic activation (PA) using the pig model were examined. Five approaches were taken to explore the function of FGF9 during in vitro embryo production (IVP). First, the maturation medium was supplemented with exogenous FGF9 to investigate its effects on maturation of GV oocytes measured by the extrusion of the first polar body hence MII oocytes. The results showed that FGF9 significantly accelerated MII rates in 50 ng/ml treated group (p<0.05) but had no visible change on embryo development after PA. However, the addition of FGF9 in both maturation and embryo culture media improved MII rates at 50 ng/ml (p<0.05), cleavage at 100 ng/ml (p<0.05) and blastocysts rate significantly (p<0.05) across treated groups after PA. Third, the addition of FGF9 in embryo culture alone revealed no visible effect. Fourth, RNA sequence analysis showed that FGF9 significantly up-regulated Thyroid hormone (TRH) providing insight into its possible mechanisms of action during IVM. With regards to oocyte quality, mitochondria membrane potential, mitochondria number and ROS levels were significantly increased at 100 ng/ml (p<0.05), reduced at 50 ng/ml (p<0.05) and increased across all FGF9-treated groups (p<0.01 and <0.05) respectively. Taken together, the present study determined that at least another thecal- and oocyte-derived product, FGF9, improves oocyte maturation and subsequent embryo development when provided during IVM and IVC. These findings, therefore, may be useful for porcine IVP system.
PHA処理がブタ体細胞核移植卵の体外発生能に及ぼす影響(Effect of phytohemagglutinin(PHA) on nuclear transfer pig embryos in vitro) , ンドゥブイシ・マチェベ; 清水聡一郎; 秦仁樹; 大畠一輝; 谷哲弥; 加藤容子 , Journal of Mammalian Ova Research , 36 , 1 , 33 , 43 , 2019年4月
概要：本研究では、ブタ単為発生卵ならびに体細胞核移植卵の体外発生能に及ぼすPHAの影響を検討した。単為発生卵ではPHA処理するとミトコンドリア活性が向上することがわかった。体細胞核移植卵では細胞融合前にPHAで5分間処理すると卵割率と胚盤胞への発生率が有意に向上した。また、ミトコンドリア関連遺伝子であるATPase6とND1の発現量が有意に増加した。以上の結果から、PHAの前処理は、ミトコンドリア活性に影響を及ぼしてブタ体細胞核移植卵の体外発生能を向上させることが示唆された。(著者抄録)
Pre-fusion treatment with phytohaemaglutinin-P (PHA-P) increases relative expression of mitochondria related genes in somatic cell nuclear transfer embryos in pig , Ndubuisi Samuel MACHEBE; Masaki HATA; Kohei ARIFUKU; Yurika NARUMIYA; Yuki ITANI; Kazuki OHATA; Tetsuya TANI; Yoko KATO , The 17th Asian-Australasian Association of Animal Production Societie , 2016年8月